產品概述:
產品參數
外觀:可溶于DMSO的灰白色固體
Ex/Em:492/517 nm (pH =9水解后)
CAS號:3348-03-6
分子式:C25H16O9
分子量:460.4
分子結構圖:

儲存條件
-20℃ 避光保存,有效期見外包裝。
產品介紹
6-CFDA 是活細胞熒光示蹤探針,具有細胞膜通透性,可以加載到細胞進行孵育。一旦進入細胞,非熒光性的 6-CFDA 由胞內酯酶水解成 6-羧基熒光素,產生熒光。這些熒光產物只能積聚在具有完整細胞膜的細胞中。因此,死細胞無完整細胞膜不能被染色。6-CFDA 標記可以通過熒光顯 微鏡或者流式細胞儀來檢測細胞。
注意事項
1. 熒光染料均存在淬滅問題,請盡量注意避光,以減緩熒光淬滅。
2. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
使用本產品的文獻:
參考文獻
1.Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51CR release assay
應用方向:細胞毒性分析
2.Tracking of leukocyte recruitment into tissUEs of mice by in situ labeling of blood cells with the fluorescent dye CFDA SE
應用方向:CFDA SE:細胞示蹤,靜脈注射標記小鼠血細胞
3.Selective labeling of embryonic neurons cultured on astrocyte monolayers with 5(6)-carboxyfluorescein diacetate (CFDA)
應用方向:標記神經元
4.Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells
應用方向:細胞增殖檢測:間充質干細胞(MSC)
5.5,6-carboxyfluorescein diacetate succinimidyl ester-labeled apoptotic and necrotic as well as detergent-treated cells can be traced in composite cell samples
應用方向:細胞分選:區分凋亡、壞死和正常細胞