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  • DAPI染色液(即用型)

    產品貨號: D4080

    產品規格: 10 mL

    目錄價(元):100

    推薦儀器:熒光顯微鏡

    大包裝詢價


產品概述:

產品參數
Ex/Em(結合DNA)=360/460 nm
CAS號:28718-90-3
分子量:350.3
分子式:C16H17Cl2N5
分子結構圖:


儲存條件
-20℃避光保存,有效期見外包裝。

產品介紹

DAPI是一種可對DNA染色的細胞核染色試劑,它在嵌入雙鏈DNA后釋放藍色熒光,亮度增強約20倍。DAPI常用于細胞凋亡檢測,染色后用熒光顯微鏡觀察或流式細胞儀檢測。DAPI具有很高的光漂白承受水平,能用來檢測酵母線粒體DNA、葉綠體DNA、病毒DNA以及染色DNA等。
以貼壁細胞(96孔板)舉例,每孔100 μL染色工作液,10 mL可以用于100個孔的染色。

注意事項

1. 使用前請將產品瞬時離心至管底,再進行后續實驗。
2. 本染色液的濃度經過優化的,可以滿足各種常規染色的需要。如需調整使用濃度,請選擇D4054,自行配置合適的工作液濃度。
3. DAPI對人體有害,請穿實驗服并戴一次性手套操作,注意適當防護。
4. 熒光染料均存在淬滅問題,請盡量注意避光,以減緩淬滅。




說明書:


 UE-D4080    

使用本產品的文獻:


1.Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin α5
Meiyu Sun, Guangfan Chi, Juanjuan Xu, Ye Tan, Jiayi Xu, Shuang Lv, Ziran Xu, Yuhan Xia, Lisha Li and Yulin Li
Stem Cell Research &Therapy (2018)
應用方向:復染

2.Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas
Shuai Jiang, Limei Qiu, Lingling Wang, Zhihao Jia, Zhao Lv, Mengqiang Wang,Conghui Liu, Jiachao Xu and Linsheng Song
Frontiers in Immunology (2018)
應用方向:復染

3.
Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a “Don’t-Eat-Us” Strategy
Yixuan Tang,Xiaoyou Wang,Jie Li,Yu Nie,Guojian Liao,Yang Yu,Chong Li
ACS NANO
應用方向:復染


4.SARS-CoV-2 productively infects human brain microvascular endothelial cells

Rui-Cheng Yang , Kun Huang, Hui-Peng Zhang , Liang Li, Yu-Fei Zhang, Chen Tan , Huan-Chun Chen, Mei-Lin Jin, Xiang-Ru Wang
Journal of Neuroinflammation


5.Synergistic effect of electrophysiological microenvironment and bioactive ions for enhancing bone regeneration
Xiaoling DengZeyu FuShengjie JiangXiaokun ChenJinjie CuiJian ZhangShengbing YangYakun LiangWeidong JiangDejian LiKaili LinXin Wang

參考文獻
1.Assay for nanogram quantities of DNA in cellular homogenates
應用方向:DNA含量測定


2.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses
應用方向:診斷細胞培養的支原體感染

3.Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
應用方向:流式細胞術中細胞核含量測定及染色體分選

4.Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer
應用方向:評估細胞凋亡


5.Direct measurement of femtogram amounts of DNA in cells and chloroplasts by quantitative microspectrofluorometry.
應用方向:免疫熒光法和原位雜交法檢測細胞核和細胞器DNA

6.SMAD2 and SMAD7 Involvement in the post-translational regulation of Muc4 via the transforming growth factor-β and interferon-γ pathways in rat mammary epithelial cells
應用方向:免疫熒光法和原位雜交法檢測細胞核和細胞器DNA

7.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses
應用方向:取代溴化乙錠對瓊脂糖凝膠中的DNA進行染色

8.Comparison of ethidium bromide and 4', 6'-diamidino-2-phenylindole as quantitative fluorescent stains for DNA in agarose gels
應用方向:取代溴化乙錠對瓊脂糖凝膠中的DNA進行染色

9.Localization of polyphosphates in Saccharomyces fragilis, as revealed by 4′, 6-diamidino-2-phenylindole fluorescence
應用方向:DAPI可與多磷酸鹽和其他聚陰離子,硫酸葡聚糖和SDS結合


10.InflUEnce of S-adenosylmethionine on 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI)-induced fluorescence of polyphosphate in the yeast vacuole
應用方向:DAPI可與多磷酸鹽和其他聚陰離子,硫酸葡聚糖和SDS結合

11.Fluorescent Complexes of DNA With DAPI 4',6-diamidine-2-phenyl indole.2HCl or DCI 4',6-dicarboxyamide-2-phenyl Indole
應用方向:DAPI可與多磷酸鹽和其他聚陰離子,硫酸葡聚糖和SDS結合

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