產品概述：

產品參數
Ex/Em（結合DNA）= 350/461 nm
Ex/Em（未結合DNA）= 346/460 nm
CAS 號：881691-13-0
分子式：C₂₇H₂₈N₆O?3HCl?3H₂O
分子量：616.0
分子結構圖：

儲存條件
-20℃避光保存，有效期見外包裝。

產品介紹
Hoechst 33342，也稱bisBenzimide H33342或HOE 33342，是一種非嵌入性的亮藍色熒光染料。染料在溶液中熒光較弱，它們在活細胞中 DNA聚AT序列富集區域的小溝處與DNA結合后熒光變得明亮，故此類染料也被稱為DNA探針。因背景較低，故染色細胞不需洗滌步驟，且染色非常穩定，對活細胞無毒，結合DNA染色后可持續幾天或更長時間。Hoechst 33342與Hoechst 33258相比在水中的溶解度要低，但兩種染料均具有高細胞膜滲透性，被廣泛用于細胞凋亡檢測，染色后可用熒光顯微鏡觀察或流式細胞儀檢測。
以貼壁細胞（96孔板）舉例，每孔100 μL染色工作液，10 mL可以用于100個孔的染色。

說明書：

 UE-H4079    

使用本產品的文獻：

1.Folic acid-modified lactoferrin nanoparticles coated with a laminarin layer loaded curcumin with dual-targeting for ulcerative colitis treatment
Naijing Ye , Peng Zhao , Shibu Ayue , Shanshan Qi , Yan Ye , Haoqi He , Linxin Dai , Ruifeng Luo , Degui Chang , Fei Gao
International Journal of Biological Macromolecules

參考文獻
1.Flow Cytometric Localization Within the Cell Cycle and Isolation of Viable Cells Following Exposure to Cytotoxic Agents
應用方向：DNA損傷識別

2.Vitality Measurement Using Spectrum Shift in Hoechst 33342 Stained Cells
應用方向：光譜偏移法測定細胞活力

3.“Liquidless” cell staining by dye diffusion from gels and analysis by laser scanning cytometry: Potential application at microgravity conditions in space
應用方向：核染色

4.4‐Hydroxynonenal Induces Oxidative Stress and Death of Cultured Spinal Cord Neurons
應用方向：觀察細胞的形態學特征：核濃縮和DNA片段化

5.Antioxidant effects of mast cell inhibitors in a human conjunctival cell line
應用方向：DNA縮合

6.Cross-linking of surface IgM in the Burkitt's lymphoma cell line ST486 provides protection against arsenite- and stress-induced apoptosis that is mediated by ERK and phosphoinositide 3-kinase signaling pathways
應用方向：與PI雙重熒光染色分析細胞凋亡

7.Pituitary Tumor Transforming Gene Causes Aneuploidy and p53-dependent and p53-independent Apoptosis
應用方向：檢測細胞凋亡

8.Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
應用方向：鑒定活細胞和凋亡細胞